Health Canada has revised the non-prescription status of vitamin D from 1000 International Units (IU) per day to 2500 IU (62.5 µg) per day effective February, 24, 2021. This applies to both cholecalciferol (D3) and ergocalciferol (D2). This follows requests from consumers, physicians and industry to increase the non-prescription limit along with many product licence applications for vitamin D with higher doses. Additionally, the volume of scientific research on this vitamin has greatly increased since the previous restriction initiated in 1997. Safety assessments were performed by Health Canada’s Food Directorate to determine the safety of vitamin D at 2500 IU and it was determined that this dose is safe for children 9 years and older, adolescents and adults. The upper tolerable intake level remains to be set at 4000 IU per day for these populations. The new maximum dosage of 2500 IU is not recommended for all subpopulations including children, adults older than 70, and those pregnant and lactating with specific dosages outlined in the document.